Single-Cell Signaling Analysis of Tumor Microenvironment Organoids

12 December 2023

Speaker:
Chris Tape
Cell Communication Lab
University College London Cancer Institute

Patient-derived organoids (PDOs) have revolutionized our ability to model personalized therapy responses. However, the current screening technologies fall short in revealing crucial drug response mechanisms and how tumor microenvironment cells influence therapeutic outcomes.

Watch this webinar to learn about:
- Single-cell multi-omics analysis: Learn how highly multiplexed mass cytometry can measure post-translational modification signaling, DNA damage, cell-cycle activity and apoptosis in over 2,500 colorectal cancer PDOs and cancer-associated fibroblasts (CAFs) at single-cell resolution.
- Role of CAFs: Learn how CAFs can regulate PDO plasticity, shifting proliferative colonic stem cells to slow-cycling revival colonic stem cells to protect cancer cells from chemotherapy.
- Commonalities in drug effects: Learn how on-target cell-cycle blockage and DNA damage drug effects are more common than previously thought, even in chemorefractory PDOs.